Cholesterol Oxidase antibody (Biotin)

Catalog No. ABIN97251

Supplier Product No.: 200-106-213-0100

The Goat Polyclonal anti-Cholesterol Oxidase antibody has been validated for ELISA, WB, IP and DB. It is suitable to detect Cholesterol Oxidase in samples from Streptomyces.

Quick Overview for Cholesterol Oxidase antibody (Biotin) (ABIN97251)

Target: Cholesterol Oxidase (choS)

Reactivity: Streptomyces

Host: Goat

Clonality: Polyclonal

Conjugate: This Cholesterol Oxidase antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Product Details anti-Cholesterol Oxidase Antibody (Biotin)

Supplier Product No.: 200-106-213-0100

Supplier: Rockland

Purpose: Cholesterol Oxidase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].

Characteristics: Synonyms: goat anti-Cholesterol Oxidase Antibody biotin Conjugation, biotin Conjugated goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

Purification: Anti-Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Cholesterol Oxidase [Streptomyces]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Cholesterol Oxidase has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Cholesterol Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Cholesterol Oxidase

Target: Cholesterol Oxidase (choS)

Alternative Name: choA

Background: Background: Anti-Cholesterol Oxidase antibody is a member of the GMC oxidoreductase family. Cholesterol Oxidase acts as a catalyst in the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of new product. Anti-Cholesterol Oxidase is ideal for investigators interested in Metabolism, Signal Transduction, or Cardiovascular research.

UniProt: P12676